Mouse Osteoblastic Cell Line (MC3T3-E1) Expresses Extracellular Calcium (Ca2+o)–Sensing Receptor and Its Agonists Stimulate Chemotaxis and Proliferation of MC3T3-E1 Cells

• DOI: 10.1359/jbmr.1998.13.10.1530
• Publication Date: 2006-04-26T19:25:23Z
• Authors: Toru Yamaguchi; Naibedya Chattopa...; Olga Kifor; Robert R. Butters; Toshitsugu Sugimoto; Edward M. Brown
• Keywords: 0301 basic medicine; Osteoblasts; Chemotaxis; Gadolinium; Neomycin; Receptors, Cell Surface; Blotting, Northern; Polymerase Chain Reaction; Cell Line; Molecular Weight; Mice; 03 medical and health sciences; Animals; Calcium; Bone Remodeling; RNA, Messenger; Receptors, Calcium-Sensing; Cell Division

The calcium-sensing receptor (CaR) is a G protein-coupled that plays key roles in extracellular calcium ion (Ca2+o) homeostasis parathyroid gland and kidney. Osteoblasts appear at sites of osteoclastic bone resorption during remodeling the "reversal" phase following preceding formation. Bone produces substantial local increases Ca2+o could provide signal for osteoblasts vicinity, leading us to determine whether such express CaR. In this study, we used mouse osteoblastic, clonal cell line MC3T3-E1. Both immunocytochemistry Western blot analysis, using an antiserum specific CaR, detected CaR protein MC3T3-E1 cells. We also identified transcripts cells by Northern analysis CaR-specific riboprobe reverse transcription-polymerase chain reaction with primers, followed nucleotide sequencing amplified products. Exposure high (up 4.8 mM) or polycationic agonists, neomycin gadolinium (Gd3+), stimulated both chemotaxis DNA synthesis Therefore, taken together, our data strongly suggest osteoblastic possesses mRNA very similar, if not identical, those Furthermore, these play role regulating turnover stimulating proliferation migration as result release Ca2+o.