Stimulation of Osteoblastic Cell Differentiation by Notch
0301 basic medicine
Cytoplasm
Osteoblasts
Green Fluorescent Proteins
Intracellular Signaling Peptides and Proteins
Bone Morphogenetic Protein 2
Membrane Proteins
Bone Marrow Cells
Cell Differentiation
Receptors, Cell Surface
Recombinant Proteins
Luminescent Proteins
Mice
03 medical and health sciences
Transforming Growth Factor beta
Bone Morphogenetic Proteins
Animals
Humans
Receptor, Notch2
Receptor, Notch1
Cells, Cultured
Transcription Factors
DOI:
10.1359/jbmr.2002.17.2.231
Publication Date:
2006-04-27T04:16:38Z
AUTHORS (7)
ABSTRACT
Abstract Notch is a transmembrane protein that plays critical role in the determination of cellular differentiation pathways. Although its importance development mesenchymal tissues has been suggested, skeletal not well investigated. Northern blot experiments showed expression Notch1 MC3T3-E1 osteoblastic cells at early stages. When cytoplasmic domain (Notch-IC [NIC]) delivered by an adenovirus vector was expressed cells, significant increase calcified nodule formation observed long-term cultures. Activation endogenous coculturing them with Delta-like-1 (Dll1)-expressing myeloma also resulted stimulation formation. Not only affecting formation, activation had effects on multipotent cells. Osteoblastic C3H10T1/2 induced bone morphogenetic 2 (BMP-2) significantly stimulated, whereas adipogenic suppressed strongly, resulting dominant NIC primary human marrow stem (hMSCs) both spontaneous and stimulated cell differentiation. These observations suggest regulated positively could be unique interesting target molecule for treatment osteoporosis.
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