Toward fast malaria detection by secondary speckle sensing microscopy

• DOI: 10.1364/boe.3.000991
• Publication Date: 2012-04-18T13:44:22Z
• Authors: Dan Cojoc; Sara Finaurini; Pavel Livshits; Eran Gur; Alon Shapira; Vicente Mico; Zeev Zalevsky
• Keywords: Blood smear; Diagnosis of malaria; Gold standard (test)

Diagnosis of malaria must be rapid, accurate, simple to use, portable and low cost, as suggested by the World Health Organization (WHO). Despite recent efforts, gold standard remains light microscopy a stained blood film. This method can detect parasitemia identify different species Plasmodium. However, it is time consuming, requires well trained microscopist good instrumentation minimize misinterpretation, thus costs are considerable. Moreover, equipment cannot easily transported installed. In this paper we propose new technique named "secondary speckle sensing microscopy" (S(3)M) based upon extraction correlation statistics patterns generated while illuminating red cells with laser inspecting them under microscope. Then, using fuzzy logic ruling principle component analysis, quality separation between healthy infected was demonstrated in preliminary experiments. The proposed used for automated high rate detection cells.