Application of fluorescence lifetime imaging microscopy to monitor glucose metabolism in pancreatic islets in vivo

• DOI: 10.1364/boe.493722
• Publication Date: 2023-07-12T15:00:09Z
• Authors: Zhongying Wang; Maani Archang; Tatyana Gurlo; Elaine Wong; Scott E. Fraser; Peter C. Butler
• Keywords: Pancreatic Islets; Carbohydrate Metabolism; Hexokinase; Glucokinase

Glucose stimulated insulin secretion is mediated by glucose metabolism via oxidative phosphorylation generating ATP that triggers membrane depolarization and exocytosis of insulin. In stressed beta cells, remodeled, with enhanced glycolysis uncoupled from phosphorylation, resulting in the impaired glucose-mediated characteristic diabetes. Relative changes can be monitored living cells using 3-component fitting approach fluorescence lifetime imaging microscopy (FLIM). We engrafted pancreatic islets onto iris to permit vivo FLIM monitoring trajectory metabolism. The results show increased islet (∼90% cells) response hyperglycemia; contrast red blood traversing maintained exclusive as expected absence mitochondria.