Handheld multispectral fluorescence lifetime imaging system for in vivo applications

• DOI: 10.1364/boe.5.000921
• Publication Date: 2014-02-26T17:59:00Z
• Authors: Shuna Cheng; Rodrigo M. Cuenca; Boang Liu; Bilal H. Malik; Joey M. Jabbour; Kristen C. Maitland; John Wright; Yi-Shing Lisa Cheng; Javier A. Jo
• Keywords: Fluorescence-lifetime imaging microscopy; Ex vivo; Cheek pouch; Indocyanine Green; Endoscope

There is an increasing interest in the application of fluorescence lifetime imaging (FLIM) for medical diagnosis. Central to clinical translation FLIM technology development compact and high-speed clinically compatible systems. We present a handheld probe design consisting small maneuverable box fitted with rigid endoscope, capable continuous at multiple emission bands simultaneously. The system was characterized using standard fluorescent dyes. performance then further demonstrated by hamster cheek pouch vivo, oral mucosa tissue both ex vivo all safe permissible exposure levels. Such can greatly facilitate evaluation cancer vivo.