Technology of the photobiostimulation of the brain’s drainage system during sleep for improvement of learning and memory in male mice

• DOI: 10.1364/boe.505618
• Publication Date: 2023-11-24T15:00:05Z
• Authors: Oxana Semyachkina...; Ivan Fedosov; Alexey Zaikin; Vasily Ageev; Egor Ilyukov; Dmitry Myagkov; Dmitry Tuktarov; Inna Blokhina; Alexander Shirokov; Andrey Terskov; Daria Zlatogorskaya; Viktoria Adushkina; Arina Evsukova; Alexander Dubrovsky; Maria Tsoy; Valeria Telnova; Maria Manzhaeva; Alexander Dmitrenko; Valeria Krupnova; Jürgen Kurths
• Keywords: 0301 basic medicine; 03 medical and health sciences; 610; 600; Article

In this study on healthy male mice using confocal imaging of dye spreading in the brain and its further accumulation in the peripheral lymphatics, we demonstrate stronger effects of photobiomodulation (PBM) on the brain’s drainage system in sleeping vs. awake animals. Using the Pavlovian instrumental transfer probe and the 2-objects-location test, we found that the 10-day course of PBM during sleep vs. wakefulness promotes improved learning and spatial memory in mice. For the first time, we present the technology for PBM under electroencephalographic (EEG) control that incorporates modern state of the art facilities of optoelectronics and biopotential detection and that can be built of relatively cheap and commercially available components. These findings open a new niche in the development of smart technologies for phototherapy of brain diseases during sleep.