Combined ion beam irradiation platform and 3D fluorescence microscope for cellular cancer research

• DOI: 10.1364/boe.522969
• Publication Date: 2024-03-11T15:00:18Z
• Authors: Andrew D. Harken; Naresh T. Deoli; Citlali Perez Campos; Brian Ponnaiya; Guy Garty; Grace S. Lee; Malte J. Casper; Shikhar Dhingra; Wenze Li; Gary W. Johnson; Sally A. Amundson; Peter W. Grabham; Elizabeth M. C. H...; David J. Brenner
• Keywords: 0301 basic medicine; 570; Medical Sciences; Eye Diseases; 610; Diseases; Medical Biochemistry; Article; Biomedical Informatics; Ophthalmology; 0302 clinical medicine; Oncology; Medical Molecular Biology; Medical Specialties; Medicine and Health Sciences

To improve particle radiotherapy, we need a better understanding of the biology of radiation effects, particularly in heavy ion radiation therapy, where global responses are observed despite energy deposition in only a subset of cells. Here, we integrated a high-speed swept confocally-aligned planar excitation (SCAPE) microscope into a focused ion beam irradiation platform to allow real-time 3D structural and functional imaging of living biological samples during and after irradiation. We demonstrate dynamic imaging of the acute effects of irradiation on 3D cultures of U87 human glioblastoma cells, revealing characteristic changes in cellular movement and intracellular calcium signaling following ionizing irradiation.