Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate

• DOI: 10.1364/boe.8.003132
• Publication Date: 2017-06-01T19:35:13Z
• Authors: Matthias Eibl; Sebastian Karpf; Daniel Weng; Hubertus Hakert; Tom Pfeiffer; Jan Philip Kolb; Robert Huber
• Keywords: Fluorescence-lifetime imaging microscopy

Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific 3-D sensing modality providing valuable information about the microstructure, composition and function of sample. However, more widespread application this technique hindered by need for sophisticated ultra-short pulse laser source speed limitations current FLIM detection systems. To overcome these limitations, we combined robust sub-nanosecond fiber as excitation with high analog bandwidth detection. Due to long length in our configuration, photons are generated per pulse, which allows us derive single only. In paper, show quality images acquired at pixel rate 1 MHz. This approach promising candidate an easy-to-use benchtop system make available wider research community.