Label-free fiber optic optrode for the detection of class C β-lactamases expressed by drug resistant bacteria

• DOI: 10.1364/boe.8.005191
• Publication Date: 2017-10-23T17:00:54Z
• Authors: Simona Zuppolini; Giuseppe Quero; Marco Consales; Laura Diodato; Patrizio Vaiano; Alberto Venturelli; Matteo Santucci; Francesca Spyrakis; Maria P. Costi; Michele Giordano; Antonello Cutolo; Andrea Cusano; Anna Borriello
• Keywords: Optode; Boronic acid

This paper reports the experimental assessment of an automated optical assay based on label free fiber optrodes for fast detection class C β-lactamases (AmpC BLs), actually considered as one most important sources resistance to β-lactams antibiotics expressed by resistant bacteria. Reflection-type long period gratings (RT-LPG) have been used highly sensitive optrodes, while a higher affine boronic acid-based ligand was here selected enhance overall performances compared those obtained in our first demonstration. In order prove feasibility analysis towards fully assay, engineered system developed simultaneously manipulate and interrogate multiple optic different phases assay. The tested AmpC solutions at increasing concentrations demonstrated limit (LOD) 6 nM, three times better when with results previous work. Moreover, real effectiveness proposed has also confirmed complex matrices case lysates Escherichia coli overexpressing AmpC.