High-speed widefield photoacoustic microscopy of small-animal hemodynamics

• DOI: 10.1364/boe.9.004689
• Publication Date: 2018-09-07T20:46:12Z
• Authors: Bangxin Lan; Wei Liu; Ya-chao Wang; Junhui Shi; Yang Li; Song Xu; Huaxin Sheng; Qifa Zhou; Jun Zou; Ulrike Hoffmann; Wei Yang; Junjie Yao
• Keywords: Frame rate

Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked high imaging speed and/or large field of view, impeding the study highly dynamic physiologic and pathophysiologic processes over region interest. Here we report high-speed system with an ultra-wide enabled by innovative water-immersible hexagon-mirror scanner. By driving scanner high-precision DC motor, new achieved cross-sectional frame rate 900 Hz 12-mm scanning range, which is 3900 times faster than our motor-scanner-based 10 MEMS-scanner-based system. Using this hexagon-scanner-based system, have imaged epinephrine-induced vasoconstriction whole mouse ear vascular reperfusion after ischemic stroke cortex vivo , spatial resolution volumetric speed. We expect that will powerful for small animal where responses view are