Wavelength agile multi-photon microscopy with a fiber amplified diode laser

• DOI: 10.1364/boe.9.006273
• Publication Date: 2018-11-15T21:40:23Z
• Authors: Matthias Eibl; Daniel Weng; Hubertus Hakert; Jan Philip Kolb; Tom Pfeiffer; Jennifer E. Hundt; Robert Huber; Sebastian Karpf
• Keywords: Narrowband; Two-photon excitation microscopy

Multi-photon microscopy is a powerful tool in biomolecular research.Less complex and more cost effective excitation light sources will make this technique accessible to broader community.Semiconductor diode seeded fiber lasers have proven be especially robust, low easy use.However, their wavelength tuning range often limited, so only limited number of fluorophores can accessed.Therefore, different approaches been proposed extend the spectral coverage these lasers.Recently, we showed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS) harnessed red-shift high power pulses from 1064 nm narrowband output at 1122 1186 therefore fluorophores.In contribution, show applicability all three wavelengths for multi-photon analyze performance.