Diffraction phase and fluorescence microscopy

• DOI: 10.1364/oe.14.008263
• Publication Date: 2006-09-08T20:53:50Z
• Authors: YongKeun Park; Gabriel Popescu; Kamran Badizadegan; Ramachandra R. Da...; Michael S. Feld
• Keywords: Interference microscopy; Phase imaging; Fluorescence-lifetime imaging microscopy; Digital Holographic Microscopy

We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative imaging epi-fluorescence investigation of live cells. The DPF instrument consists an interference microscope, which is incorporated into conventional inverted microscope. images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to cell lifetime. potential the quantifying rapid nanoscale motions in cells demonstrated experiments on red blood cells, while composite phase-fluorescence mode exemplified with mitotic kidney