Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement biosensors

• DOI: 10.1364/oe.16.021626
• Publication Date: 2008-12-16T14:43:44Z
• Authors: Nikhil Ganesh; Ian D. Block; Patrick C. Mathias; Wei Zhang; Edmond Chow; Viktor Malyarchuk; Brian T. Cunningham
• Keywords: Electronic Data Processing; Optics and Photonics; Tumor Necrosis Factor-alpha; Biosensing Techniques; Equipment Design; 02 engineering and technology; Models, Theoretical; Microscopy, Atomic Force; Fluorescence; Immunoenzyme Techniques; Spectrometry, Fluorescence; Quantum Dots; Animals; 0210 nano-technology; Fluorescent Dyes; Oligonucleotide Array Sequence Analysis

Efficient recovery of light emitted by fluorescent molecules employing photonic structures can result in high signal-to-noise ratio detection for biological applications including DNA microarrays, fluorescence microscopy and single molecule detection. By a model system comprised colloidal quantum dots, we consider the physical basis extraction effect as provided crystals. Devices with different lattice symmetry are fabricated ensuring spectral spatial coupling dot emission leaky eigenmodes characteristics studied using angle-resolved angle-integrated measurements. Comparison numerical calculations lifetime measurements reveals that enhancement occurs via resonant redirection radiation. various lattices differences factor maximum approaching 220. We also demonstrate first enhanced biosensor allows over 20-fold signal cytokine TNF-alpha sandwich immunoassay.