A detection instrument for enhanced-fluorescence and label-free imaging on photonic crystal surfaces

• DOI: 10.1364/oe.17.013222
• Publication Date: 2009-07-20T11:35:34Z
• Authors: Ian D. Block; Patrick C. Mathias; Nikhil Ganesh; Sarah I. Jones; Brian R. Dorvel; Vikram Chaudhery; Lila O. Vodkin; Rashid Bashir; Brian T. Cunningham
• Keywords: Biomolecule; Fluorescence-lifetime imaging microscopy

We report on the design and demonstration of an optical imaging system capable exciting surface-bound fluorophores within resonant evanescent electric field a photonic crystal surface gathering fluorescence emission that is directed toward objective by crystal. The also has ability to quantify shifts in local resonance angle induced adsorption biomolecules for label-free biomolecular imaging. With these two capabilities combined single detection system, we demonstrate images self-registered enhanced with 328x more sensitive relative glass surface. This technique applied DNA microarray where quantification immobilized capture enables improved quality control subsequent dye-tagged hybridized yields 3x genes be detected versus commercially available substrates.