ImFCS: A software for Imaging FCS data analysis and visualization

• DOI: 10.1364/oe.18.025468
• Publication Date: 2010-11-23T15:48:51Z
• Authors: Jagadish Sankaran; Xianke Shi; Liang Yoong Ho; Ernst H. K. Stelzer; Thorsten Wohland
• Keywords: Fluorescence Correlation Spectroscopy; Optical sectioning; Point spread function

The multiplexing of fluorescence correlation spectroscopy (FCS), especially in imaging FCS using fast, sensitive array detectors, requires the handling large amounts data. One can easily collect excess 100,000 curves a day, too many to be treated manually. Therefore, ImFCS, an open-source software which relies on standard image files was developed and provides wide range options for calculation spatial temporal auto- cross-correlations, as well differences Cross-Correlation Functions (ΔCCF). ImFCS permits fitting models functions optimized histograms fitted parameters. Applications include measurement diffusion flow with Imaging Total Internal Reflection (ITIR-FCS) Single Plane Illumination Microscopy (SPIM-FCS) biologically relevant samples. As compromise between ITIR-FCS SPIM-FCS, we extend applications Variable Angle-FCS (IVA-FCS) where sub-critical oblique illumination sample sectioning close cover slide.