Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging

• DOI: 10.1364/oe.21.014555
• Publication Date: 2013-06-11T17:04:49Z
• Authors: Shuo Pang; Chao Han; Jessey Erath; Ana Rodriguez; Changhuei Yang
• Keywords: 610; Breast Neoplasms; Equipment Design; Image Enhancement; 530; 01 natural sciences; Equipment Failure Analysis; Microscopy, Fluorescence, Multiphoton; Cell Line, Tumor; 0103 physical sciences; Humans; Lighting

The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm(2) at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.