Color-multiplexed 3D differential phase contrast microscopy with optimal annular illumination

• DOI: 10.1364/oe.545480
• Publication Date: 2024-12-13T08:00:10Z
• Authors: Jingfan Wang; Xing Zhao; Yan Wang; Da Li
• Keywords: Interference microscopy

Quantitative phase imaging (QPI) has become a valuable tool in the field of biomedical research due to its ability quantify refractive index variations live cells and tissues. For example, three-dimensional differential contrast (3D DPC) uses through-focus images captured under different illumination patterns deconvoluted with computed 3D transfer function (PTF) reconstruct index. In conventional DPC semi-circular illumination, partially spatially coherent often diminishes contrast, exacerbating inherent noise, can lead large number zero values PTF, resulting strong low-frequency artifacts deteriorating resolution. To overcome above drawbacks, we obtain conditions for acquiring optimal PTF based on analysis model derivation calculation process propose microscopy annular illumination. The proposed pattern minimizes missing frequency components Fourier space, best noise-robustness significantly increased contrast. expedite speed, utilize 1/2 multiplexed reducing data acquisition volume by 75%. tomography simulated object, unstained tongue sections, oral epithelial demonstrates that our method achieves advantages. conclusion, demonstrate novel microscope only requires replacing commercial programmable LED array. accurate compactness system setup allow play significant role field.