Optical scatter imaging: subcellular morphometry in situ with Fourier filtering

• DOI: 10.1364/ol.26.001063
• Publication Date: 2007-08-17T15:28:40Z
• Authors: Nada N. Boustany; Scot C. Kuo; Nitish V. Thakor
• Keywords: Spatial frequency; Point spread function; Numerical aperture; Spatial filter; Aperture (computer memory)

We demonstrate a quantitative optical scatter imaging (OSI) technique, based on Fourier filtering, for detecting alterations in the size of particles with wavelength-scale dimensions. generate our image by taking ratio images collected at high and low numerical aperture central dark-field microscopy. Such an spatially encodes wide to narrow angle hence provides measure local particle size. validated OSI sphere suspensions live cells. In cells, revealed biochemically induced morphological changes that were not apparent unprocessed differential interference contrast images. Unlike high-resolution methods, can provide information smaller than camera's spatial resolution.