High-speed three-dimensional random access scanning with a linear SLM

• DOI: 10.1364/optica.536853
• Publication Date: 2024-10-30T13:01:16Z
• Authors: Caroline Berlage; Urs L. Böhm; Ana Sanchez Moreno; Julia Ledderose; Albert Gidon; Matthew E. Larkum; Andrew Plested; Benjamin Judkewitz

High-speed volumetric imaging is crucial for observing fast and distributed processes such as neuronal activity. Multiphoton microscopy helps to mitigate scattering effects inside tissue, but the standard raster scanning approach limits achievable volume rates. Random access point can lead a considerable speed-up by sampling only pre-selected locations, existing techniques based on acousto-optic deflectors are still limited rate of up . This number parallel targets at high acquisition rates necessary, example, in voltage or synaptic events. Here, we introduce SPARCLS, method 3D random 340 kHz using single 1D phase modulator. We show potential this events with fluorescent glutamate sensors mammalian organotypic slices well zebrafish larvae.