High-speed phase aberration correction in digital holographic microscopy via vortex-Legendre method

• DOI: 10.1364/opticaopen.28215728
• Publication Date: 2025-01-16T07:21:19Z
• Authors: Carlos Trujillo; Karina Ortega; René Restrepo; Alfonso Padilla-V...; Ana Doblas
• Keywords: Digital Holographic Microscopy; Digital holography

Digital holographic microscopy (DHM) is an effective technique for quantitative phase imaging (QPI) of microscopic samples, yet its accuracy hinges on precise compensation wavefront aberrations. Conventional methods often rely iterative, time-intensive procedures or additional optical elements, making it difficult to rapidly and accurately generate fully compensated maps. This work introduces a numerical vortex as efficient method automatically correcting the tilt aberration introduced in off-axis DHM. In addition, combining vortices with robust implementation Legendre polynomial fitting addresses residual higher-order errors. combined vortex-Legendre markedly improves background flatness reconstructed maps, achieving average slope 1.8×10−4 rad/pixel across entire imaged field view. Validation using calibrated objects, such USAF test targets star targets, well biological samples (e.g., red blood cells), demonstrates effectiveness reducing computational overhead approximately 1 second conventional laptop while maintaining high measurement quality 960×1280-px2 holograms. automated represents significant advancement real-time, high-accuracy DHM applications