An Improved Methodology to Evaluate Cell and Molecular Signals in the Reparative Callus During Fracture Healing

• DOI: 10.1369/0022155419900915
• Publication Date: 2020-01-11T09:16:35Z
• Authors: Anuradha Valiya K...; Justin N. Williams; Uma Sankar
• Keywords: Bone decalcification; Callus

Approximately 5% to 10% of all bone fractures do not heal completely, contributing significant patient suffering and medical costs. Even in healthy individuals, fracture healing is associated with downtime loss productivity. However, no pharmacological treatments are currently available promote efficient healing. A better understanding the underlying molecular mechanisms crucial for developing novel therapies hasten The early reparative callus that forms around site injury a fragile tissue consisting shifting cell populations held together by loose connective tissue. delicate challenging section vulnerable disintegration during harsh steps immunostaining, namely, decalcification, deparaffinization, antigen retrieval. Here, we describe an improved methodology processing early-stage calluses immunofluorescence labeling sections visualize temporal (timing) spatial (location) patterns cellular events regulate This method has short turnaround time from sample collection microscopy as it does require lengthy decalcification. It preserves structural integrity entail deparaffinization or methods Our can be adapted high-throughput screening drugs efficacious healing: