Aberrant Gata-3 Expression in Human Pancreatic Cancer

• DOI: 10.1369/jhc.5a6626.2005
• Publication Date: 2005-08-09T00:24:58Z
• Authors: Antanas Gulbinas; Pascal O. Berberat; Zilvinas Dambrauskas; Thomas Giese; Nathalia Giese; Frank Autschbach; Joerg Kleeff; Stefan Meuer; Markus W. Büchler; Helmut Friess
• Keywords: Adult; Aged, 80 and over; Cell Nucleus; Male; 0301 basic medicine; Cytoplasm; Microscopy, Confocal; Electrophoretic Mobility Shift Assay; GATA3 Transcription Factor; Middle Aged; Immunohistochemistry; 3. Good health; Pancreatic Neoplasms; Protein Transport; 03 medical and health sciences; 0302 clinical medicine; Cell Line, Tumor; Humans; Female; RNA, Messenger; 10. No inequality; Pancreas; Aged; Neoplasm Staging; Signal Transduction

Gata-3 has been shown to specifically alter its expression patterns in different types of cancers. Recent evidence suggests that an interference exists the TGF-beta signaling pathway. To determine role pancreatic cancer, cancer samples were analyzed comparison normal tissues. Furthermore, four cell lines with alterations pathway studied. evaluate if a potential relationship exists, we correlated mRNA levels TGF-betas, receptors, and Smad-3. Finally, influence on vitro. All demonstrated marked overexpression protein. Immunohistochemical staining revealed strong persistent cytoplasmic immunoreactivity cells. In electrophoretic mobility shift assay, disturbed nuclear translocation was confirmed. The showed significant correlation responsive downregulation upon exposure, whereas TGF-beta-unresponsive lines, persisted at high levels. specific upregulation impaired cooperative action pathway, suggesting plays central human cancer.