LIM Kinase 2 Is Widely Expressed in All Tissues

• DOI: 10.1369/jhc.5c6813.2006
• Publication Date: 2006-01-07T01:58:45Z
• Authors: Karla Acevedo; Nathalie Moussi; Rong Li; Priscilla Soo; Ora Bernard
• Keywords: Cofilin; Immunofluorescence

The LIM kinase family includes two proteins: LIMK1 and LIMK2. These proteins have identical genomic structure overall amino acid identity of 50%. Both regulate actin polymerization via phosphorylation inactivation the depolymerizing factors ADF/cofilin. Although function endogenous is well established, little known about LIMK2 protein. To understand specific role protein, we examined its expression in embryonic adult mice using a rat monoclonal antibody, which recognizes specifically PDZ domain but not that LIMK1. Immunoblotting immunoprecipitation analyses mouse tissues human cell lines revealed widespread 75-kDa Immunofluorescence analysis demonstrated cellular localization different from protein found cytoplasm localized to punctae enriched within focal adhesions like Immunohistochemical studies widely expressed pattern similar except testes. We also form heterodimers, does always interact with same as (J Histochem Cytochem 54:487-501, 2006)