Correction: Structural Characterization of the Complex of SecB and Metallothionein-Labeled proOmpA by Cryo-Electron Microscopy

• DOI: 10.1371/annotation/b4828aa9-5156-499b-9f30-a8f9c9dd4f8e
• Publication Date: 2013-05-24T13:46:13Z
• Authors: Qiang Zhou; Shan Sun; Phang Tai; Sen-Fang Sui
• Keywords: Cryo-Electron Microscopy; Metallothionein; Characterization

ProOmpA is a preprotein that translocated across the plasma membrane by general secretory pathway in Escherichia coli.The molecular chaperon SecB Sec can recognize and bind proOmpA for its translocation.However, structure of SecB/proOmpA complex remains unknown.Here, we constructed an uncleavable fused with metallothionein at C-terminus labeled it metals vitro study cryo-electron microscopy.Using single particle microscopy, reconstructed 3D stable complex.The shows major portion locates on one side tetramer, resulting asymmetric binding pattern.This work also provides possible approach to determination small protein complexes cryoelectron microscopy.