The Fifth Adaptor Protein Complex

• DOI: 10.1371/journal.pbio.1001170
• Publication Date: 2011-10-11T21:40:48Z
• Authors: Jennifer Hirst; Lael D. Barlow; Gabriel Casey Fra...; Daniela A. Sahlender; Matthew N. J. Seaman; Joel B. Dacks; Margaret S. Robinson
• Keywords: Transport protein; Clathrin adaptor proteins; Sorting nexin

Adaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP have been identified, which are present in most eukaryotes. We report existence a fifth complex, AP-5. Tagged AP-5 localises late endosomal HeLa cells. does not associate with clathrin and is insensitive brefeldin A. Knocking down subunits interferes trafficking cation-independent mannose 6-phosphate receptor causes form swollen structures emanating tubules. can be found all five eukaryotic supergroups, but they co-ordinately lost many organisms. Concatenated phylogenetic analysis provides robust resolution, first time, evolutionary order emergence adaptor subunit families, showing AP-3 as basal followed by AP-5, AP-4, AP-1 AP-2. Thus, an evolutionarily ancient involved sorting, has links hereditary spastic paraplegia.