Midgut-derived neuropeptide F controls germline stem cell proliferation in a mating-dependent manner

• DOI: 10.1371/journal.pbio.2005004
• Publication Date: 2018-09-24T17:41:00Z
• Authors: Tomotsune Ameku; Yuto Yoshinari; Michael J. Texada; Shu Kondo; Kotaro Amezawa; Goro Yoshizaki; Yuko Shimada-Niwa; Ryusuke Niwa
• Keywords: Receptors, Neuropeptide; 0301 basic medicine; QH301-705.5; Enteroendocrine Cells; Cell Count; Models, Biological; Sexual Behavior, Animal; 03 medical and health sciences; Animals; Drosophila Proteins; Biology (General); Cell Proliferation; Base Sequence; Stem Cells; Neuropeptides; Ovary; Ecdysteroids; Drosophila melanogaster; Germ Cells; Bone Morphogenetic Proteins; Female; Digestive System; Cell Division; Research Article; Signal Transduction

Stem cell maintenance is established by neighboring niche cells that promote stem self-renewal. However, it poorly understood how activity regulated systemic, tissue-extrinsic signals in response to environmental cues and changes physiological status. Here, we show neuropeptide F (NPF) signaling plays an important role the pathway regulating mating-induced germline (GSC) proliferation fruit fly Drosophila melanogaster. NPF expressed enteroendocrine (EECs) of midgut released seminal-fluid protein sex peptide (SP) upon mating. This midgut-derived controls GSC via ovarian receptor (NPFR) activity, which modulates bone morphogenetic (BMP) levels GSCs. Our study provides a molecular mechanism describes gut-derived systemic factor couples behavior status, such as mating, through interorgan communication.