Regulation of glial size by eicosapentaenoic acid through a novel Golgi apparatus mechanism

• DOI: 10.1371/journal.pbio.3001051
• Publication Date: 2020-12-28T23:19:58Z
• Authors: Albert Zhang; Ziqiang Guan; Kyle Ockerman; Pengyuan Dong; Jiansheng Guo; Zhiping Wang; Dong Yan
• Keywords: 0301 basic medicine; QH301-705.5; Ubiquitin-Protein Ligases; Golgi Apparatus; 03 medical and health sciences; Eicosapentaenoic Acid; Fatty Acids, Unsaturated; Animals; Biology (General); Caenorhabditis elegans; Caenorhabditis elegans Proteins; Neuroglia; Research Article; Cell Size

Coordination of cell growth is essential for the development brain, but molecular mechanisms underlying regulation glial and neuronal size are poorly understood. To investigate involved in regulation, we used Caenorhabditis elegans amphid sheath (AMsh) glia as a model show that conserved cis -Golgi membrane protein eas-1/GOLT1B negatively regulates growth. We found eas-1 inhibits E3 ubiquitin ligase rnf-145 /RNF145, which, turn, promotes nuclear activation sbp-1/ SREBP, key regulator sterol fatty acid synthesis, to restrict At early developmental stages, network sbp-1 promote glia, when animals reach adult stage, this inhibition released through an eas-1- dependent shuttling from trans stop Furthermore, identified long-chain polyunsaturated acids (LC-PUFAs), especially eicosapentaenoic (EPA), downstream products eas-1-rnf-145-sbp-1 pathway functions prevent overgrowth glia. Together, our findings reveal novel potentially mechanism control.