Structural organization and energy storage in crosslinked actin assemblies

• DOI: 10.1371/journal.pcbi.1006150
• Publication Date: 2018-05-29T13:41:00Z
• Authors: Rui Ma; Julien Berro
• Keywords: Elastic energy; Actin remodeling

During clathrin-mediated endocytosis in yeast cells, short actin filaments (< 200nm) and crosslinking protein fimbrin assemble to drive the internalization of plasma membrane. However, organization meshwork during remains largely unknown. In addition, only a small fraction force necessary elongate pinch off vesicles can be accounted for by polymerization alone. this paper, we used mathematical modeling study self-organization rigid presence elastic crosslinkers conditions relevant endocytosis. We found that condense into either disordered or an ordered bundle depending on filament length mechanical kinetic properties crosslinkers. Our simulations also demonstrated these nanometer-scale structures store large amount energy within (up 10kBT per crosslinker). This conversion binding is consequence geometric constraints created helical pitch filaments, which results frustrated configurations attached filaments. propose stored at later time endocytic process. As proof principle, presented simple mechanism sustained torque production detachment from pair parallel