CIRCUST: A novel methodology for temporal order reconstruction of molecular rhythms; validation and application towards a daily rhythm gene expression atlas in humans

• DOI: 10.1371/journal.pcbi.1011510
• Publication Date: 2023-09-28T17:45:25Z
• Authors: Yolanda Larriba; Ivy C. Mason; Richa Saxena; Frank A. J. L. Sc...; Cristina Rueda
• Keywords: Expression (computer science)

The circadian system drives near-24-h oscillations in behaviors and biological processes. underlying core molecular clock regulates the expression of other genes, it has been shown that more than 50 percent genes mammals displays 24-h rhythmic patterns, with specific cycle varying from one tissue to another. Determining gene patterns human tissues sampled as single timepoints several challenges, including reconstruction temporal order highly noisy data. Previous methodologies have attempted address these challenges or a small number for which evolutionary conservation is assumed be preserved. Here we introduce CIRCUST, novel CIRCular-robUST methodology analyzing rhythms, relies on circular statistics, robust against noise, requires fewer assumptions existing methodologies. Next, validated method four controlled experiments sampling times were known, finally, CIRCUST was applied 34 Genotype-Tissue Expression (GTEx) dataset aim towards building comprehensive daily rhythm atlas humans. validation application here indicate provides flexible framework formulate solve issues related analysis rhythms tissues. publicly available at https://github.com/yolandalago/CIRCUST/ .