Genome-Wide Analysis of Factors Affecting Transcription Elongation and DNA Repair: A New Role for PAF and Ccr4-Not in Transcription-Coupled Repair

• DOI: 10.1371/journal.pgen.1000364
• Publication Date: 2009-02-07T04:15:24Z
• Authors: Hélène Gaillard; Cristina Tous; Javier Botet; Cristina González...; Maria José Quintero; Laia Viladevall; María L. García-R...; Alfonso Rodríguez...; Antonio Marín; Joaquín Ariño; José Luis Revuelta; Sebastián Chávez; Andrés Aguilera
• Keywords: RNA polymerase II; Transcription; General transcription factor

RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for resumption. One important type hindrance consists DNA lesions, which are by transcription-coupled repair (TC-NER), specific sub-pathway nucleotide excision repair. To improve our knowledge and its coupling TC-NER, we used the yeast library non-essential knock-out mutations screen genes conferring resistance transcription-elongation inhibitor mycophenolic acid DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence subunits SAGA Ccr4-Not complexes, Mediator, Bre1, Bur2, Fun12 affect different extents. Given dependency TC-NER on Polymerase II fact few proteins known involved in related transcription, performed an in-depth analysis selection mutants. We found mutants PAF complexes impaired TC-NER. This study provides required efficient yeast, unraveling novel function these opening new perspectives understanding functional interconnection elongation.