The Baker's Yeast Diploid Genome Is Remarkably Stable in Vegetative Growth and Meiosis

• DOI: 10.1371/journal.pgen.1001109
• Publication Date: 2010-09-09T19:17:50Z
• Authors: K. T. Nishant; Wu Wei; Eugenio Mancera; Juan Lucas Argueso; Andreas Schlattl; Nicolas Delhomme; Xin Ma; Carlos D. Bustamante; Jan O. Korbel; Zhenglong Gu; Lars M. Steinmetz; Eric Alani
• Keywords: Subtelomere; Mating of yeast

Accurate estimates of mutation rates provide critical information to analyze genome evolution and organism fitness. We used whole-genome DNA sequencing, pulse-field gel electrophoresis, comparative hybridization determine in diploid vegetative meiotic accumulation lines Saccharomyces cerevisiae. The underwent only mitotic divisions while the a cycle every ∼20 divisions. Similar base substitution were estimated for both lines. Given our experimental design, these measures indicated that rate is within range being equal zero 55-fold higher than rate. Mutations detected all heterozygous those homozygous. A quantitative analysis intra-tetrad mating events showed inter-spore primarily responsible rapidly fixing mutations homozygosity as well removing mutations. did not observe 1–2 nt insertion/deletion (in-del) any sequenced one structural variant non-telomeric location was found. However, large number variations subtelomeric sequences seen affect viability. Our results indicate yeast nuclear remarkably stable during cell cycles support hypothesis peripheral regions chromosomes are more dynamic gene-rich central sections where rearrangements could be deleterious. This work also provides an improved estimate mutational load carried by organisms.