Interactions among Polycomb Domains Are Guided by Chromosome Architecture

• DOI: 10.1371/journal.pgen.1001343
• Publication Date: 2011-03-24T20:54:29Z
• Authors: Bas Tolhuis; Marleen Blom; Ron M. Kerkhoven; Ludo Pagie; Hans Teunissen; Marja Nieuwland; Marieke Simonis; Wouter de Laat; Maarten van Lohuizen; Bas van Steensel
• Keywords: Chromosome conformation capture

Polycomb group (PcG) proteins bind and regulate hundreds of genes. Previous evidence has suggested that long-range chromatin interactions may contribute to the regulation PcG target Here, we adapted Chromosome Conformation Capture on Chip (4C) assay systematically map chromosomal in Drosophila melanogaster larval brain tissue. Our results demonstrate genes interact extensively with each other nuclear space. These are highly specific for genes, because non-target either low or high expression show distinct interactions. Notably, mostly limited same chromosome arm, a topological rather than sequence-based mechanism is responsible this constraint. many among exist these guided by overall architecture.