SHINE Transcription Factors Act Redundantly to Pattern the Archetypal Surface of Arabidopsis Flower Organs

• DOI: 10.1371/journal.pgen.1001388
• Publication Date: 2011-05-26T21:06:14Z
• Authors: Jian Xin Shi; Sergey Malitsky; Sheron De Oliveira; Caroline Branigan; Rochus B. Franke; Lukas Schreiber; Asaph Aharoni
• Keywords: Cutin; Petal; Abscission; Epidermis (zoology); Cuticle (hair)

Floral organs display tremendous variation in their exterior that is essential for organogenesis and the interaction with environment. This diversity surface characteristics largely dependent on composition structure of coating cuticular layer. To date, mechanisms flower organ initiation identity have been studied extensively, while little known regarding regulation formation, cuticle composition, its developmental significance. Using a synthetic microRNA approach to simultaneously silence three SHINE (SHN) clade members, we revealed these transcription factors act redundantly shape morphology Arabidopsis flowers. It appears SHNs regulate floral organs' epidermal cell elongation decoration nanoridges, particularly petals. Reduced activity SHN results fusion earlier abscission accompanied by decrease cutin load modified wall properties. possess target genes within four cutin- suberin-associated protein families including, CYP86A cytochrome P450s, fatty acyl-CoA reductases, GSDL-motif lipases, BODYGUARD1-like proteins. The suggest alongside controlling lipids metabolism, modify epidermis through altering pectin metabolism structural We also provide evidence formation petals other during growth or dehiscence partially mediated gibberellin DELLA signaling cascade. study therefore demonstrates need defined order form archetypal features surfaces control cell-to-cell separation processes. Furthermore, it will promote future investigation into relation between patterning broader development biological functions.