A Gene Regulatory Network for Root Epidermis Cell Differentiation in Arabidopsis

• DOI: 10.1371/journal.pgen.1002446
• Publication Date: 2012-01-12T22:08:54Z
• Authors: Angela Bruex; Raghunandan M. Ka...; Yana Wieckowski; Yeon Hee Kang; Christine Bernhardt; Yang Xia; Xiaohua Zheng; Jean Y. Wang; Myeong Min Lee; Philip Benfey; Peter J. Woolf; John Schiefelbein
• Keywords: Gene regulatory network; Epidermis (zoology); Root hair

The root epidermis of Arabidopsis provides an exceptional model for studying the molecular basis cell fate and differentiation. To obtain a systems-level view epidermal differentiation, we used genome-wide transcriptome approach to define organize large set genes into transcriptional regulatory network. Using mutants that produce only one two types, together with fluorescence-activated cell-sorting preferentially analyze transcriptome, identified 1,582 differentially expressed in root-hair or non-hair including 208 "core" genes. organization core network was accomplished by using 17 distinct 2 hormone treatments perturb system assess effects on each gene's transcript accumulation. In addition, temporal gene expression information from developmental time series dataset predicted associations derived Bayesian modeling were aid positioning within Further, detailed functional analysis likely bHLH network, MYC1, bHLH54, bHLH66, bHLH82, showed three subfamilies proteins participate development stage-specific manner. integration genetic, genomic, computational analyses new composition, architecture, logic it demonstrates utility comprehensive systems dissecting complex