Cell Lineage Analysis of the Mammalian Female Germline

• DOI: 10.1371/journal.pgen.1002477
• Publication Date: 2012-02-23T21:57:00Z
• Authors: Yitzhak Reizel; Shalev Itzkovitz; Rivka Adar; Judith Elbaz; Adrian Jinich; Noa Chapal-Ilani; Yosef E. Maruvka; Nava Nevo; Zipora Marx; Inna Horovitz; Adam Wasserstrom; Avi Mayo; Irena Shur; Dafna Benayahu; Karl Skorecki; Eran Segal; Nava Dekel; Ehud Shapiro
• Keywords: Lineage (genetic); Cell lineage

Fundamental aspects of embryonic and post-natal development, including maintenance the mammalian female germline, are largely unknown. Here we employ a retrospective, phylogenetic-based method for reconstructing cell lineage trees utilizing somatic mutations accumulated in microsatellites, to study germline dynamics mice. Reconstructed can be used estimate relationships between different types, as well depth (number divisions since zygote). We show that, reconstructed mouse trees, oocytes form clusters that separate from hematopoietic mesenchymal stem cells, both young old mice, indicating these populations belong distinct lineages. Furthermore, while cumulus cells sampled ovarian follicles distinctly clustered on left right ovaries not, suggesting mixing their progenitor pools. also observed an increase oocyte with age, which explained either by depth-guided selection ovulation or renewal. Overall, our sheds light substantial novel preservation development.