A Systematic Analysis of Cell Cycle Regulators in Yeast Reveals That Most Factors Act Independently of Cell Size to Control Initiation of Division

• DOI: 10.1371/journal.pgen.1002590
• Publication Date: 2012-03-15T21:50:38Z
• Authors: Scott A. Hoose; Jeremy A. Rawlings; Michelle M. Kelly; M. Camille Leitch; Qotaiba O. Ababneh; Juan P. Robles; David Taylor; Evelyn M. Hoover; Bethel Hailu; Kayla A. McEnery; S. Sabina Downing; Deepika Kaushal; Yi Chen; Alex Rife; Kirtan A. Brahmbhatt; Roger Smith; Michael Polymenis
• Keywords: 0301 basic medicine; 2. Zero hunger; Homozygote; DNA; Saccharomyces cerevisiae; QH426-470; G1 Phase Cell Cycle Checkpoints; Fungal Proteins; 03 medical and health sciences; Gene Expression Regulation, Fungal; Genetics; Gene Regulatory Networks; Ribosomes; Cell Division; Gene Deletion; Research Article; Cell Proliferation; Cell Size

Upstream events that trigger initiation of cell division, at a point called START in yeast, determine the overall rates proliferation. The identity and complete sequence those remain unknown. Previous studies relied mainly on size changes to identify systematically genes required for timely completion START. Here, we evaluated panels non-essential single gene deletion strains altered DNA content by flow cytometry. This analysis revealed most deletions cycle progression did not change size. Our results highlight strong requirement ribosomal biogenesis protein synthesis division. We also identified numerous factors have been previously implicated control mechanisms. found CBS, which catalyzes cystathionine from serine homocysteine, advances two ways: promoting growth, requires CBS's catalytic activity, separate function, does require activity. CBS defects cause disease humans, animals has vital, non-catalytic, unknown roles. Hence, our may be relevant human biology. Taken together, these findings significantly expand range systematic identification regulators division describe will valuable resource yeast other organisms.