Telomerase-Null Survivor Screening Identifies Novel Telomere Recombination Regulators

• DOI: 10.1371/journal.pgen.1003208
• Publication Date: 2013-01-17T21:49:29Z
• Authors: Yan Hu; Hong-Bo Tang; Ning-Ning Liu; Xia-Jing Tong; Wei Dang; Yi-Min Duan; Xiao-Hong Fu; Yang Zhang; Jing Peng; Fei-Long Meng; Jin-Qiu Zhou
• Keywords: Telomere-binding protein; RAD52

Telomeres are protein–DNA structures found at the ends of linear chromosomes and crucial for genome integrity. Telomeric DNA length is primarily maintained by enzyme telomerase. Cells lacking telomerase will undergo senescence when telomeres become critically short. In Saccharomyces cerevisiae, a very small percentage cells can remain viable lengthening via two distinct homologous recombination pathways. These “survivor” classified as either Type I or II, with each class survivor possessing telomeric genetic requirements. To elucidate regulatory pathways contributing to generation, we knocked out RNA gene TLC1 in 280 telomere-length-maintenance (TLM) mutants examined telomere post-senescent survivors. We uncovered new functional roles 10 genes that affect emerging ratio versus II survivors 22 required generation. further verified Pif1 helicase was INO80 chromatin remodeling complex greatly affected frequency Finally, Rad6-mediated ubiquitination pathway KEOPS were recombination. Our data provide an independent line evidence supporting idea these play important dynamics.