Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions

• DOI: 10.1371/journal.pgen.1005962
• Publication Date: 2016-04-01T17:38:04Z
• Authors: Ulrike Mäder; Pierre Nicolas; Maren Depke; Jan Pané-Farré; Michel Debarbouille; Magdalena M. van ...; Cyprien Guérin; Sandra Dérozier; Aurelia Hiron; Hanne Jarmer; Aurélie Leduc; Stephan Michalik; Ewoud Reilman; Marc Schaffer; Frank Schmidt; Philippe Bessières; Philippe Noirot; Michael Hecker; Tarek Msadek; Uwe Völker; Jan Maarten van Dijl
• Keywords: Sigma factor; Transcription; General transcription factor

Staphylococcus aureus is a major pathogen that colonizes about 20% of the human population. Intriguingly, this Gram-positive bacterium can survive and thrive under wide range different conditions, both inside outside body. Here, we investigated transcriptional adaptation S. HG001, derivative strain NCTC 8325, across experimental conditions ranging from optimal growth in vitro to intracellular host cells. These data establish an extensive repertoire transcription units non-coding RNAs, classification 1412 promoters according their dependence on RNA polymerase sigma factors SigA or SigB, allow identification new potential targets for several known factors. In particular, study revealed relatively low abundance antisense RNAs aureus, where they overlap only 6% coding genes, 19 not co-transcribed with other genes were found. Promoter analysis comparison Bacillus subtilis links small number less profound impact alternative aureus. Furthermore, Rho-dependent termination suppresses pervasive transcription, presumably originating abundant spurious initiation A+T-rich genome, which would otherwise affect expression overlapped genes. summary, our provides genome-wide information regulation as well insights into biological function Rho implications bacteria.