Requirement of the 3′-UTR-dependent suppression of DAZL in oocytes for pre-implantation mouse development

• DOI: 10.1371/journal.pgen.1007436
• Publication Date: 2018-06-08T13:32:06Z
• Authors: Kurumi Fukuda; Aki Masuda; Takuma Naka; Atsushi Suzuki; Yuzuru Kato; Yumiko Saga
• Keywords: Mice, Knockout; 0301 basic medicine; Embryonic Development; Gene Expression Regulation, Developmental; RNA-Binding Proteins; QH426-470; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Oogenesis; Ovarian Follicle; Genetics; Oocytes; Animals; Female; RNA, Messenger; 3' Untranslated Regions; Research Article

Functional oocytes are produced through complex molecular and cellular processes. In particular, the contribution of post-transcriptional gene regulation mediated by RNA-binding proteins (RBPs) is crucial for controlling proper expression during this process. DAZL (deleted in azoospermia-like) one RBPs required sexual differentiation primordial germ cells progression meiosis ovulated oocytes. However, involvement development follicular still unknown. Here, we show that Dazl translationally suppressed a 3′-UTR-dependent manner oocytes, suppression normal pre-implantation development. We found occurred postnatal concomitant with formation follicles, whereas mRNA was continuously expressed throughout oocyte development, raising possibility dispensable survival growth Indeed, oocyte-specific knockout resulted production number pups. On other hand, genetically modified female mice overexpress fewer numbers pups than control due to defective Our data suggest an important mechanism functional