Dual functions for the ssDNA-binding protein RPA in meiotic recombination

• DOI: 10.1371/journal.pgen.1007952
• Publication Date: 2019-02-04T18:27:54Z
• Authors: Baolu Shi; Jiangyang Xue; Hao Yin; Rui Guo; Mengcheng Luo; Lan Ye; Qinghua Shi; Xiaoyan Huang; Mingxi Liu; Jiahao Sha; P. Jeremy Wang
• Keywords: Synapsis; Replication protein A; Chromosomal crossover; Synaptonemal complex

Meiotic recombination permits exchange of genetic material between homologous chromosomes. The replication protein A (RPA) complex, the predominant ssDNA-binding is required for nearly all aspects DNA metabolism, but its role in mammalian meiotic remains unknown due to embryonic lethality RPA mutant mice. a heterotrimer RPA1, RPA2, and RPA3. We find that loss largest subunit, leads disappearance RPA2 RPA3, resulting absence complex. Using an inducible germline-specific inactivation strategy, we completely abrogates loading RAD51/DMC1 recombinases programmed double strand breaks, thus blocking invasion chromosome pairing synapsis. Surprisingly, MEIOB, SPATA22, ATR breaks RPA-independent does not promote recruitment RPA. Finally, reduces crossover formation. Our results demonstrate plays two distinct roles recombination: essential recombinase at early stages important promoting formation later stages.