Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation
0301 basic medicine
570
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Nanog Homeobox Protein
QH426-470
Zebrafish Proteins
Chromatin Assembly and Disassembly
Chromatin
576
Genomic Imprinting
03 medical and health sciences
Genetics
Animals
Octamer Transcription Factor-3
SOX Transcription Factors
Zebrafish
Research Article
DOI:
10.1371/journal.pgen.1008546
Publication Date:
2020-01-15T18:54:27Z
AUTHORS (4)
ABSTRACT
ABSTRACTIn many organisms, early embryonic development is driven by maternally provided factors until the controlled onset of transcription during zygotic genome activation. The regulation of chromatin accessibility and its relationship to gene activity during this transition remains poorly understood. Here, we generated chromatin accessibility maps from genome activation until the onset of lineage specification. During this period, chromatin accessibility increases at regulatory elements. This increase is independent of RNA polymerase II-mediated transcription, with the exception of the hyper-transcribed miR-430 locus. Instead, accessibility often precedes the transcription of associated genes. Loss of the maternal transcription factors Pou5f3, Sox19b, and Nanog, which are known to be required for zebrafish genome activation, results in decreased accessibility at regulatory elements. Importantly, the accessibility of regulatory regions, especially when established by Pou5f3, Sox19b and Nanog, is predictive for future transcription. Our results show that the maternally provided transcription factors Pou5f3, Sox19b, and Nanog open up chromatin and prime genes for activity during zygotic genome activation in zebrafish.
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