Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation

Homeobox protein NANOG Transcription RNA polymerase II ChIA-PET
DOI: 10.1371/journal.pgen.1008546 Publication Date: 2020-01-15T18:54:27Z
ABSTRACT
In many organisms, early embryonic development is driven by maternally provided factors until the controlled onset of transcription during zygotic genome activation. The regulation chromatin accessibility and its relationship to gene activity this transition remain poorly understood. Here, we generated maps with ATAC-seq from activation lineage specification. During period, increases at regulatory elements. This increase independent RNA polymerase II-mediated transcription, exception hypertranscribed miR-430 locus. Instead, often precedes associated genes. Loss maternal Pou5f3, Sox19b, Nanog, which are known be required for zebrafish activation, results in decreased Importantly, regions, especially when established Sox19b predictive future transcription. Our show that Nanog open up prime genes zebrafish.
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