Bidirectional crosstalk between Hypoxia-Inducible Factor and glucocorticoid signalling in zebrafish larvae

• DOI: 10.1371/journal.pgen.1008757
• Publication Date: 2020-05-07T17:40:04Z
• Authors: Davide Marchi; Kirankumar Santha...; Eleanor Markham; Nan Li; Karl-Heinz Storbeck; Nils Krone; Vincent T. Cunliffe; Fredericus J. M. ...
• Keywords: Crosstalk; Hypoxia; Hypoxia-Inducible Factors; Signalling

In the last decades in vitro studies highlighted potential for crosstalk between Hypoxia-Inducible Factor-(HIF) and glucocorticoid-(GC) signalling pathways. However, how this interplay precisely occurs vivo is still debated. Here, we use zebrafish larvae (Danio rerio) to elucidate what degree hypoxic affects endogenous glucocorticoid pathway vice versa, vivo. Firstly, our results demonstrate that presence of upregulated HIF signalling, both receptor (Gr) responsiveness cortisol levels are repressed 5 days post fertilisation larvae. addition, despite activity being low at normoxia, data show it already impedes levels. Secondly, further analysed contribution glucocorticoids activity. Interestingly, (GR) mineralocorticoid (MR) play a key role enhancing it. Finally, found indications promote via multiple routes. Cumulatively, findings allowed us suggest model