SNX-3 mediates retromer-independent tubular endosomal recycling by opposing EEA-1-facilitated trafficking

• DOI: 10.1371/journal.pgen.1009607
• Publication Date: 2021-06-03T18:16:06Z
• Authors: Yangli Tian; Qiaoju Kang; Xuemeng Shi; Yuan Wang; Nali Zhang; Huan Ye; Qifeng Xu; Tao Xu; Rongying Zhang
• Keywords: Retromer; Sorting nexin; ESCRT

Early endosomes are the sorting hub on endocytic pathway, wherein nexins (SNXs) play important roles for formation of distinct membranous microdomains with different functions. Tubular mediate recycling clathrin-independent (CIE) cargoes back toward plasma membrane. However, molecular mechanism underlying tubule is still poorly understood. Here we screened effect ARF-6-associated CIE endosomal tubules all SNX members in Caenorhabditis elegans ( C . ). We identified SNX-3 as an essential factor generation tubules. The loss abolishes interconnected intestine Consequently, surface and total protein levels hTAC strongly decreased. Unexpectedly, depletion retromer components VPS-26/-29/-35 has no similar effect, implying that trimer dispensable this process. determined captured by ESCRT complex transported into lysosome rapid degradation snx-3 mutants. Interestingly, EEA-1 increasingly recruited early localized to hTAC-containing structures mutant intestines. also showed SNX3 EEA1 compete each other binding phosphatidylinositol-3-phosphate enriching Hela cells. Our data demonstrate first time PX domain-only organizes tubular efficient retrieves cargo away from maturing competing endosomes. our results call question how couples capture membrane remodeling absence complex.