Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans

• DOI: 10.1371/journal.pgen.1009714
• Publication Date: 2021-09-02T18:17:33Z
• Authors: Eric J. Foss; Smitha Sripathy; Tonibelle Gatbont...; Hyunchang Kwak; Adam H. Thiesen; Uyen Lao; Antonio Bedalov
• Keywords: Origin recognition complex; Pre-replication complex; Replication; Replication timing

The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading pre-replication complexes (pre-RCs) at onset S-phase, and differences in timing activation these during S phase. To determine degree which distribution pre-RC alone could account for chromosomal patterns, we mapped binding sites Mcm2-7 helicase complex (MCM) budding yeast, fission mouse humans. We observed similar individual MCM double-hexamer (DH) footprints species, but notable their distribution: Footprints yeast were more sharply focused compared other three organisms, consistent with relative sequence specificity origins . cerevisiae Nonetheless, some clear exceptions, most notably inactive X-chromosome, much fluctuation along chromosomes all four organisms reflected complexes.