Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans
DNA Replication
0301 basic medicine
0303 health sciences
03 medical and health sciences
Minichromosome Maintenance Proteins
Genetics
Chromosomes, Human
Humans
Saccharomyces cerevisiae
QH426-470
Chromosomes, Fungal
Genome, Fungal
Research Article
DOI:
10.1371/journal.pgen.1009714
Publication Date:
2021-09-02T18:17:33Z
AUTHORS (7)
ABSTRACT
The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S phase. To determine the degree to which distribution of pre-RC loading alone could account for chromosomal replication patterns, we mapped the binding sites of the Mcm2-7 helicase complex (MCM) in budding yeast, fission yeast, mouse and humans. We observed similar individual MCM double-hexamer (DH) footprints across the species, but notable differences in their distribution: Footprints in budding yeast were more sharply focused compared to the other three organisms, consistent with the relative sequence specificity of replication origins in S. cerevisiae. Nonetheless, with some clear exceptions, most notably the inactive X-chromosome, much of the fluctuation in replication timing along the chromosomes in all four organisms reflected uneven chromosomal distribution of pre-replication complexes.
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