A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription

• DOI: 10.1371/journal.pgen.1009889
• Publication Date: 2021-11-01T17:36:21Z
• Authors: Amandine Bonnet; Carole Chaput; Noé Palmic; Benoit Palancade; Pascale Lesage
• Keywords: Retrotransposon; Nuclear pore; Transcription

Beyond their canonical function in nucleocytoplasmic exchanges, nuclear pore complexes (NPCs) regulate the expression of protein-coding genes. Here, we have implemented transcriptomic and molecular methods to specifically address impact NPC on retroelements, which are present multiple copies genomes. We report a novel for Nup84 complex, core building block, restricting transcription LTR-retrotransposons yeast. complex-dependent repression impacts both Copia Gypsy Ty LTR-retrotransposons, all over S . cerevisiae genome. Mechanistically, complex restricts Ty1, most active yeast retrotransposon, through tethering SUMO-deconjugating enzyme Ulp1 NPCs. Strikingly, modest accumulation Ty1 RNAs caused by loss-of-function is sufficient trigger an important increase cDNA levels, resulting massive retrotransposition. Altogether, our study expands understanding interactions between retrotransposons NPC, highlights importance cells keep under tight transcriptional control.