MxB inhibits long interspersed element type 1 retrotransposition

• DOI: 10.1371/journal.pgen.1010034
• Publication Date: 2022-02-16T18:29:37Z
• Authors: Yu Huang; Fengwen Xu; Shan Mei; Xiaoman Liu; Fei Zhao; Liang Wei; Zhangling Fan; Yamei Hu; Liming Wang; Bin Ai; Shan Cen; Chen Liang; Fei Guo
• Keywords: Retrotransposon; Stress granule

Long interspersed element type 1 (LINE-1, also L1 for short) is the only autonomously transposable in human genome. Its insertion into a new genomic site may disrupt function of genes, potentially causing genetic diseases. Cells have thus evolved battery mechanisms to tightly control LINE-1 activity. Here, we report that cellular antiviral protein, myxovirus resistance protein B (MxB), restricts mobilization LINE-1. This MxB requires nuclear localization signal located at its N-terminus, GTPase activity and ability form oligomers. We further found associates with ORF1p promotes sequestration G3BP1-containing cytoplasmic granules. Since knockdown stress granule marker proteins G3BP1 or TIA1 abolishes inhibition LINE-1, conclude engages components effectively sequester within granules, hindering from accessing nucleus complete retrotransposition. Thus, provides one mechanism cells mobility retroelements.