Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure

• DOI: 10.1371/journal.pgen.1010432
• Publication Date: 2022-10-10T17:54:08Z
• Authors: Ana Novačić; Dario Menéndez; Jurica Ljubas; Slobodan Barbarić; Françoise Stutz; Julien Soudet; Igor Stuparević
• Keywords: Transcription; Transcription coregulator

Pervasive transcription of eukaryotic genomes generates non-coding transcripts with regulatory potential. We examined the effects antisense on regulation expression yeast PHO5 gene, a paradigmatic case for gene through promoter chromatin remodeling. A negative role at locus was demonstrated by leveraging level overlapping specific mutant backgrounds, from strong in cis , and use CRISPRi system. Furthermore, we showed that enhanced elongation leads to more repressive conformation promoter, which is slowly remodeled upon induction. The effect mitigated inactivation histone deacetylase Rpd3, showing RNA acts via deacetylation. This pathway Rpd3-dependent decreased recruitment RSC remodeling complex induction transcription. Overall, data this work reveal an additional mechanism transcription-mediated repression structure