PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae

• DOI: 10.1371/journal.pgen.1010593
• Publication Date: 2023-07-06T17:38:20Z
• Authors: Ellen R. Wagner; Nicole M. Nightin...; Annie Jen; Katherine A. Over...; Mick McGee; Joshua J. Coon; Audrey P. Gasch
• Keywords: Proteomics; Repressor Proteins; Xylose; Saccharomyces cerevisiae Proteins; Glucose; Fermentation; Saccharomyces cerevisiae; Anaerobiosis; Lipid Metabolism; Cyclic AMP-Dependent Protein Kinases; Lipids; Research Article

Organisms have evolved elaborate physiological pathways that regulate growth, proliferation, metabolism, and stress response. These must be properly coordinated to elicit the appropriate response an ever-changing environment. While individual been well studied in a variety of model systems, there remains much uncover about how are integrated produce systemic changes cell, especially dynamic conditions. We previously showed deletion Protein Kinase A (PKA) regulatory subunit BCY1 can decouple growth metabolism Saccharomyces cerevisiae engineered for anaerobic xylose fermentation, allowing robust fermentation absence division. This provides opportunity understand PKA signaling normally coordinates these processes. Here, we transcriptomic, lipidomic, phospho-proteomic responses upon glucose shift across series strains with different genetic mutations promoting either coupled or decoupled xylose-dependent metabolism. Together, results suggested defects lipid homeostasis limit bcy1Δ strain despite To further this mechanism, performed adaptive laboratory evolutions re-evolve parental strain. The harbored TPK1 regulator OPI1 , among other genes, profiles gene expression. Deletion opi1 partially reverted strain’s phenotype parent, reduced fermentation. suggest several models cells coordinate budding yeast restructuring processes enables utilization.