LRK-1/LRRK2 and AP-3 regulate trafficking of synaptic vesicle precursors through active zone protein SYD-2/Liprin-α

• DOI: 10.1371/journal.pgen.1011253
• Publication Date: 2024-05-09T17:39:54Z
• Authors: Sravanthi S. P. N...; Shirley B. Dixit; Neena Ratnakaran; Anushka Deb; Sneha Hegde; Sri Padma Priya B...; Sierra Swords; Barth D. Grant; Sandhya P. Koushika
• Keywords: Transport protein; LRRK2

Synaptic vesicle proteins (SVps) are transported by the motor UNC-104/KIF1A. We show that SVps travel in heterogeneous carriers C . elegans neuronal processes, with some SVp co-transporting lysosomal (SV-lysosomes). LRK-1/LRRK2 and clathrin adaptor protein complex AP-3 play a critical role sorting of away from each other at SV-lysosomal intermediate trafficking compartment. Both lacking SV-lysosomes dependent on UNC-104/KIF1A for their transport. In lrk-1 mutants, both can axons absence UNC-104, suggesting LRK-1 plays an important to enable UNC-104 transport synaptic proteins. Additionally, acts upstream regulates its membrane localization. complex, become more UNC-104-SYD-2/Liprin-α Therefore, SYD-2 link events likely through interaction UNC-104. further mistrafficking into dendrite apb-3 mutants depends SYD-2, regulating recruitment AP-1/UNC-101. concert AP complexes ensure polarized & SVps.