Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast

• DOI: 10.1371/journal.pgen.1011509
• Publication Date: 2024-12-09T18:38:11Z
• Authors: Anna Marešová; Michaela Grulyová; Miluše Hradilová; Viacheslav Zemlia...; Jarmila Princová; Martin Převorovský
• Keywords: Transcription; Schizosaccharomyces

Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides powerful platform to study the intricate regulatory mechanisms governing these fundamental processes. In S. pombe, Cbf11 Mga2 proteins transcriptional activators of non-sterol metabolism genes, with also known as regulator. Despite sharing common set target little was about their functional relationship. This reveals that function together in same pathway, critical for mitotic fidelity. Deletion either gene results similar array defects, including slow growth, dysregulated homeostasis, impaired (cut phenotype), abnormal morphology, perturbed transcriptomic proteomic profiles, compromised response stressors camptothecin thiabendazole. Remarkably, double deletion mutant does not exhibit more severe phenotype compared single mutants. addition, ChIP-nexus analysis bind nearly identical positions within promoter regions genes. Interestingly, binding appears be dependent on presence likely acts tether DNA, while is needed activate explores distribution homologs across fungi. Basidiomycota contrasts Ascomycota, which mostly lack but retain Mga2. suggests an evolutionary rewiring circuitry conclusion, this offers compelling support functioning jointly regulate fidelity yeast.